LyoCoN by Martin Christ. Optimisation of freezing for vials

LyoCoN Single Vial initiated crytallisation via Martin Christ from John Morris Scientific

LyoCoN from John Morris Scientific

picture 1: ice crystal accumulation at ice condenser

Freezing of many vials in a freeze dryer normally works out as a stochastic process. All vials will freeze only within a certain time frame, e.g. 1 hour. Furthermore, this crystallisation happens at different temperatures. Crystal structure and later drying behaviour will diverge.

The newly developed technology in LyoCoN creates a controlled nucleation of allvials to be lyophilised: After loading of the machine, the cold ice condenser accumulates ice crystals (pict. 1). Simultaneously the liquid product is cooled down close to the freezing point. In the next step a slight vacuum is established in the freeze dryer (pict. 2). The external recipient is kept under atmospheric pressure. Finally a pressure equilibration between recipient and freezedryer is started by opening a valve to the ice condenser.

LyoCoN from John Morris Scientific

picture 2: formation of a slight vacuum in the freeze dyrer

The air/gas from the recipient is injected into the ice condenser. The resulting ice fog infiltrates all vials (pict. 3). These crystal nuclei immediately start homogenious crystallisation in all vials.

After this, the freezedryer is aerated completely to atmospheric pressure. Freezing of all crystallized vials can be continued in a conventional way, e.g. further freezing down, annealing etc.

Martin Christ LyoCoN – facts at a glance:

  • formation of the ice fog with moisture out of the product, no external media necessary. GMP-compliant!

    LyoCoN picture 3 from John Morris Scientific

    picture 3: injection of an ice fog in the product chamber by pressure compensation between external recipient and ice condenser chamber

  • simple basic principle – vacuum available anyhow. No overpressure design of the machine necessary
  • therefor also ideal for smaller pilot units + retrofit easy possible
  • no external waste media – which might contain drug/product – produced by LyoCoN.



VIDEO: Just a button push initiates crytallisation of all vials inside freezedryer.

VIDEO: LyoCoN-demonstration in a production unit Epsilon 2-65D.

To find out more about Martin Christ Laboratory Freeze Dryers,
please contact our Vacuum experts
Email: or
Free call AUS. 1800 251 799 and NZ  0800 651 700

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